Hibernation Omics Analysis Workflows

Interactive visualization pipelines for seasonal metabolic reprogramming research.
From sequence alignment to multi-omics integration, specialized for torpor-arousal cycles.

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3. scRNA-seq

Single-cell RNA-seq

Cell-type specific metabolic quiescence & reactivation

Hibernation Focus: Cell cycle arrest (G0/G1), adipocyte browning trajectory, hypoxia-inducible states
1
Cross-season Integration
Harmony/SCVI Batch Correction Active vs Torpor
2
Cell Cycle & Metabolic Scoring
Seurat G0 Arrest OXPHOS/Glycolysis
3
Trajectory Inference
Monocle3 Adipocyte Browing RNA Velocity
4
Cell-Cell Communication
CellChat Leptin/Adiponectin Seasonal LR Pairs
Recommended Tools
UMAP/PCA Violin Plot Cell Fate Flow RNA Velocity Heatmap